Effect of proteins in serum was estimated by comparing MALDI-ToF mass spectra of amino acids-spiked serum before and after the methanol extraction. Parylene-matrix chip was applied to MALDI-ToF MS analysis reducing the matrix peaks significantly at low mass-to-charge ratio range (m/z  0.98) and the LODs were ranging from 9.0 to 22.9 μg/mL. Newborn screening for diagnosis of phenylketonuria, homocystinuria, and maple syrup urine disease have been conducted by analyzing the concentration of target amino acids using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF MS) based on parylene-matrix chip. Kim, Jo-Il Noh, Joo-Yoon Kim, Mira Park, Jong-Min Song, Hyun-Woo Kang, Min-Jung Pyun, Jae-Chul Newborn screening by matrix-assisted laser desorption/ionization mass spectrometry based on parylene-matrix chip.
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